Several sterilization methods for CO2 and carbon dioxide incubators

A carbon dioxide incubator is a device that simulates the formation of a growth environment similar to that of cells/tissues in an organism. The incubator requires a stable temperature (37 ° C), a stable CO2 level (5%), a constant pH (7.2-7.4), and a high relative saturation humidity (95%) to cultivate cells/tissues in vitro. It is an instrument for cell, tissue, and bacterial culture, and is used for immunology Key equipment necessary for oncology, genetics, and biotechnology.

The main factor in the design of anti pollution and disinfection and sterilization systems is the destruction of different devices for cell culture and production to reduce and prevent the occurrence of pollution, mainly by reducing the growth of microorganisms and surface areas that can be produced, combined with automatic purification devices to effectively prevent pollution.

For example, considering that carbon dioxide incubators are accompanied by mold growth during use, various sterilization methods have emerged to ensure that the carbon dioxide incubator is not contaminated and the biological instruments inside the incubator are clean,

1. The ultraviolet disinfection function of the carbon dioxide incubator;

2. The design and production of HEPA filters can filter air inside trains. It can filter out over 99.97% of particles with a diameter of 0.3 microns. In addition, it is designed to kill microbial contamination in hot and humid environments, achieving the purpose of sterilization and disinfection.

These devices are necessary for cell culture. When choosing a cleaning device, we first consider the sterilization ability of various methods. The UV disinfection ability is inversely proportional to the square of the distance between the UV lamp and the target. The farther the distance, the worse the disinfection ability. Therefore, the UV disinfection method has its limitations and is difficult to achieve the requirements of complete sterilization; HEPA filters, due to the influence of membrane pore size, are unable to remove BD and some small bacteria, and also have their limitations; Compared to other methods, high-temperature disinfection is currently a more effective method of disinfection and sterilization. High temperature disinfection can be divided into two categories: traditional high-temperature dry heat disinfection and high-temperature wet heat sterilization.