The Dry Microbial Penetration Resistance Tester (DMPT) is a testing device used to assess the resistance of medical clothing, such as surgical gowns or protective coveralls, to the penetration of microbial contaminants. This test helps evaluate the effectiveness of the clothing in preventing the transmission of pathogens during medical procedures or in high-risk environments.
The DMPT test method typically involves the following steps:
1. Test Specimen Preparation: A representative sample of the medical clothing is selected for testing. The specimen is carefully prepared as per the specified dimensions and requirements outlined in the testing standard or protocol.
2. Microbial Challenge: The challenge microorganism, usually a non-pathogenic surrogate organism, is applied to the outer surface of the test specimen. The microorganism is typically in the form of a suspension or a dried droplet, simulating potential contamination scenarios.
3. Drying Time: After applying the microbial suspension, the test specimen is allowed to dry for a specific duration. This step aims to replicate real-world conditions where the clothing may encounter moisture exposure and subsequent drying.
4. Recovery Plate Preparation: A culture medium, such as agar, is prepared in Petri dishes. These plates will be used to collect any microorganisms that penetrate the clothing and reach the other side.
5. Assembly and Testing: The test specimen is securely mounted onto the DMPT apparatus, ensuring a proper seal to prevent leakage. The assembly is placed in an incubation chamber that maintains the desired temperature and humidity levels. Airborne microbial challenge may also be introduced into the chamber to simulate exposure to microbial aerosols.
6. Incubation and Evaluation: The test specimen is incubated for a specified period, allowing any penetrating microorganisms to grow on the recovery plates. Afterward, the plates are analyzed for microbial colony formation. The number and location of colonies indicate the extent of microbial penetration through the clothing.
The Dry Microbial Penetration Resistance Tester provides a standardized and controlled method for assessing the barrier properties of medical clothing against microbial contamination. It helps manufacturers, regulatory bodies, and healthcare facilities understand the level of protection provided by different types of clothing and make informed decisions regarding their selection and use in various healthcare settings.
It is important to note that specific testing procedures, equipment, and evaluation criteria may vary depending on the testing standard or protocol followed, such as ASTM F1670/F1670M or ISO 22612. It is advisable to consult the relevant standard to obtain precise testing requirements and instructions.